Objective: To establish a UPLC-MS-MS analysical method for simultaneous determination of concentrations of 2

3

5

4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside

l-deoxynojirimycin

cassiaside

rubrofusarin gentiobioside

aurantio-obtusin

chryso-obtusin

resveratrol in rat plasma and to study their pharmacokinetic characteristics after taking Zishen Qinggan Daiping formula in rats. Method: Acidified plasma samples were precipitated for protein with methanol.Waters Acquity UPLC BEH C18 column was employed with mobile phase of 0.1% formic acid-water and acetonitrile for gradient elution.Detection was carried out by multiple reaction monitoring (MRM) positive ion mode with ESI ionization source. Result: Seven constituents showed a good linear relationship with extraction recovery ranging between 94.83%-106.58% and a high intra-and inter-day precisions and accuracy.Their pharmacokinetic parameters in model group rats were AUC0-∞ of (326.65±26.66) μg·L-1·h-1

t1/2 of (3.64±1.69) h

Tmax of 0.33 h

CLz/F of (60.56±5.32) L·h-1·kg-1

Vz/F of (325.13±167.18) L·kg-1

Cmax of (169.25±18.02) μg·L-1. Conclusion: This mentioned method is specific

rapid

sensitive and suitable for pharmacokinetic studies of Zishen Qinggan Daiping formula in rats.