Objective: To optimize the most effective component formula of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma on hepatoma carcinoma

and to reveal its antitumor mechanisms. Method: Human hepatoma cell SMMC-7721 was used as object and human normal liver cell line L-O2 was used as control in this study. The inhibiting effect on SMMC-7721 and the protective effect on L-O2 were performed by CCK-8 assay

and the most effective component formula of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma was optimized by using the orthogonal design method. The effect on SMMC-7721 and L-O2 viabilities were assayed by using Real-time cell assay and the time-effect relationship was analyzed. Cell apoptosis was measured by high content screening using Annexin V/PI double staining. Result: The results demonstrated that the contents of total salvianolic acid

total saponins Ginseng Radix et Rhizoma and ginseng polysaccharide doses were 10

10

5 mg·L-1

respectively

in the optimizing component formula of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma . Furthermore

the component formula of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma could significantly decrease the SMMC-7721 cell viability and induce SMMC-7721 cell apoptosis

while there was no significant difference on the normal liver cell. Conclusion: The component formula of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma has potentially specific and selective anti-hepatoma carcinoma effect. Its mechanism may be related to the inhibition of hepatoma cell proliferation and induction of cell apoptosis.