Objective: To observe the role of Atractylodis Rhizoma extract on protecting gastric mucosa and gastrointestinal dysfunction of rats with spleen deficiency syndrome

and to elucidate the mechanism of Atractylodis Rhizoma extract regulating spleen deficiency syndrome. Method: Male rats were fed with Xiaochengqi decoction and hunger to establish spleen deficiency syndrome models.Then the animal were randomly divided into model group

Atractylodes Rhizome extract high

middle and low dose group(20

10

5 g·kg-1)

Domperidone group 5 mg·kg-1. After 10 days of treatment

charcoal gavage method was used o determine residual rate of stomach and small intestinal propulsion ratio of the rats

hematoxylin and eosin(HE) staining was used to observe the gastric mucosa pathological and histological changes

like gastric mucosa tissue cells of glandular stomach area and ultrastructural alterations of intercellular junctions with transmission electron microscopy method

laser Doppler blood flow meter was used to measure gastric mucosal blood flow

immunohistochemical was used to determinate the expression of trefoil factor 1(TFF1) in gastric mucosa and c-kit expression in colon tissue volume. Result: Compared with the normal group

gastric mucosal morphology had significantly abnormal change in model group;gastric mucosal blood flow

gastric mucosa TFF1 and colon tissue volume c-kit expression were decreased (P < 0.01);residual rate of stomach was increased and small intestinal propulsion ratio was decreased(P < 0.05

P < 0.01). After intervention of Atractylodis Rhizoma extract

those indicators were improved(P<0.05

P<0.01). Conclusion: Atractylodis Rhizoma extract can inhibit the gastric mucosal damage of spleen deficiency rat

protect and repair the damaged mucous membrane organization

and improve the gastrointestinal function of spleen deficiency rats.