吸波面积-HPLC结合法测定白花蛇舌草组分胶囊中整体和个体成分溶出度

胡悦; 赖宏强; 范哲贤; 姜梦丽; 李孝栋

doi:10.13422/j.cnki.syfjx.2015140004

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吸波面积-HPLC结合法测定白花蛇舌草组分胶囊中整体和个体成分溶出度

药剂与炮制 | 更新时间：2024-01-04

- 吸波面积-HPLC结合法测定白花蛇舌草组分胶囊中整体和个体成分溶出度
- Dissolution of Total Composition and Individual Material in Components Capsules of by Combination of Area Under Absorbance-wavelength Curve and HPLC
- 中国实验方剂学杂志 2015年21卷第14期页码：4-7
- 作者机构：
- 作者简介：
- 基金信息：
  
  福建省科技平台建设项目(2010Y2004)
- DOI：10.13422/j.cnki.syfjx.2015140004
  中图分类号： O657.72;R284
- 纸质出版日期：2015
- 稿件说明：
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胡悦, 赖宏强, 范哲贤, 等. 吸波面积-HPLC结合法测定白花蛇舌草组分胶囊中整体和个体成分溶出度[J]. 中国实验方剂学杂志, 2015,21(14):4-7.

HU Yue, LAI Hong-qiang, FAN Zhe-xian, et al. Dissolution of Total Composition and Individual Material in Components Capsules of by Combination of Area Under Absorbance-wavelength Curve and HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 4-7.
胡悦, 赖宏强, 范哲贤, 等. 吸波面积-HPLC结合法测定白花蛇舌草组分胶囊中整体和个体成分溶出度[J]. 中国实验方剂学杂志, 2015,21(14):4-7. DOI： 10.13422/j.cnki.syfjx.2015140004.

HU Yue, LAI Hong-qiang, FAN Zhe-xian, et al. Dissolution of Total Composition and Individual Material in Components Capsules of by Combination of Area Under Absorbance-wavelength Curve and HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 4-7. DOI： 10.13422/j.cnki.syfjx.2015140004.

摘要

目的: 建立吸波面积-HPLC结合法测定白花蛇舌草组分胶囊中整体和个体成分溶出度的方法。方法: 以含0.5%十二烷基硫酸钠的pH 6.8磷酸盐缓冲液为溶出介质

转速100 r ·min-1

采用紫外-可见分光光度法和HPLC对混合药物及个体成分进行含量测定

比较测定结果的差异。结果: 在120 min时

吸波面积法测得整体混合药物的累积溶出率74.72%

HPLC测得槲皮素、齐墩果酸和豆甾醇在同等时间段的累积溶出率分别为77.16%

82.78%

71.70%;吸波面积法与HPLC计算的累积溶出量分别为104.61

102.25 mg。结论: 2种方法具有较好的一致性

吸波面积-HPLC结合法可用于白花蛇舌草组分胶囊中整体和个体药物成分溶出度的测试

为其他复方制剂中不同药物成分的溶出度研究提供借鉴。

Abstract

Objective: To establish a method for determining dissolution of total composition and individual material in components capsules of Hedyotis diffusa by combination of area under absorbance-wavelength curve (AUAWC) and high performance liquid chromatography (HPLC). Method: Dissolution medium was pH 6.8 phosphate buffer containing 0.5% sodium dodecyl sulfate (SDS) with a rotation speed of 100 r · min-1.Total composition and individual material was respectively analyzed by UV-Vis spectrophotometry and HPLC for content determination

data were compared scientifically. Result: Cumulative dissolution of total composition at 120 min by AUAWC was 74.72%

cumulative dissolution of quercetin

oleanolic acid and stigmasterol by HPLC at the same time was 77.16%

82.78% and 71.70%

respectively.Final dissolution quantity of total composition by AUAWC and total quantity of three individual drugs by HPLC were 104.61 mg and 102.25 mg

respectively. Conclusion: Data under two methods have a good consistency.Combination of AUAWC and HPLC can be used for dissolution test of total composition and individual ingredient from components capsule

which will provide a good instruction for dissolution of different components from other preparations.

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