Objective: To optimize extraction process of total polysaccharides from Artemisiae Annuae Herba residue and investigate its antioxidant activity. Method: Response surface methodology was employed to optimize extraction process of total polysaccharides from Artemisiae Annuae Herba residue by taking liquid-solid ratio

extracting temperature and time as factors.Antioxidant activity of total polysaccharides was evaluated through several in vitro antioxidant assays

such as 1

1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity

hydroxyl radical scavenging activity and anti-lipid peroxidation with vitamin C as a reference. Result: Optimum extraction conditions of total polysaccharides were as follows:liquid-solid ratio of 30: 1

extraction time of 30 min and extraction temperature of 70 ℃.Under these conditions

yield of total polysaccharides could be up to 1.773 3%.Antioxidant capacity of total polysaccharides increased with the concentration of total polysaccharides increased

its scavenging ability towards hydroxyl free radical was stronger than vitamin C. Conclusion: Optimized extraction process is stable and reliable

total polysaccharides from Artemisiae Annuae Herba residue has antioxidant capacity

which can provide a reference for comprehensive utilization of Artemisiae Annuae Herba.