Objective: To separate and purificate homogeneous polysaccharides from Phellodendri Amurensis Cortex and study the effect on proliferation function of mice spleen lymphocyte transformation. Method: The DEAE Cellulose 52

DEAE-Sepharose F.F and Sephacryl S-400 columns were used to separate and purificate homogeneous polysaccharides and the monosaccharide composition and structure were preliminarily identificated by periodate oxidation

smith degradation

methylation

IR

HPLC and GC-MS

and their effects on proliferation function of mice spleen lymphocyte transformation were determinated by MTT. Result: The yield of crude polysaccharide was 4%. Three homogeneous polysaccharides were separated and purificated (G1-G3)

the yields of purified polysaccharides were 6%

9%

and 10%

espectively

which were identified as homogeneous polysaccharides by HPLC. They mainly contained arabinose

rhamnose

galactose and galacturonic acid

the mole ratios were 1: 1.06: 1.04: 1.14 (G1)

1: 1.27: 1.10: 1.09 (G2) and 1: 1.23: 0.99: 1.55 (G3)

and the homogeneous polysaccharides were able to inhibit the proliferation of lymphocytes induced by ConA. Conclusion: The homogeneous polysaccharides contained arabinose

rhamnose

galactose and galacturonic acid

and showed inhibit effect of cellular immunity

which established the experimental basis for the further study of the polysaccharides.