Objective: To establish the quality standard for Houerhuan anti-inflammation granule. Method: The gallic acid was qualitative identified by TLC

the contents of gallic acid and quercetin were determined by HPLC. HPLC was performed on Odyssil C18 column (4.6 mm×250 mm

5 μm)

gradiently eluting with acetonitrile as mobile phase A

and 0.2%H3PO4 as mobile phase B at a flow rate of 1.0 mL · min-1. The column temperature was 30 ℃. The detection wavelength was at 254 nm. The injection volume was 5 μL. Result: TLC spots were clear

separation effect was good. The linearity of gallic acid was good in the range of 0.060 2-1.806 μg (r=0.999 5)

and the average recovery was 99.88% (RSD 1.8%). The linearity of quercetin was good in the range of 0.016 9-0.507 6 μg(r=0.999 6)

and the average recovery was 100.24%(RSD 1.9%). Conclusion: The established method is simple

accurate and provides reference for quality control of Houerhuan anti-inflammation granule.