Objective: To observe the effect of Tiaoganshen Qutanyu-formula (TQF) on blood pressure and kidney tissue injury index on the early stage of spontaneous hypertensive rats (SHR) administered before blood pressure peak. Method: A total of 30 12-week male SHR were randomly divided into 3 groups:the TQF group (4 g · kg-1)

the losartan group (30 mg · kg-1) and the model group. Another healthy 10 SD rats were taken as the normal group. The blood pressure was observed. Microalbuminuria (mALb) and β2-urinary microglobulin (β2-MG) were detected by ELISA after 12 weeks of treatment. The serum contents of creatinine (SCr) and urea nitrogen (BUN) were observed. Moreover

collagen I and collagen IV in kidney tissues were detected by using immunohistochemical method. Result: Compared with the normal group

the blood pressure elevated

the levels of mAlb

β2-MG

BUN and SCr increased

the collagen I and collagen IV in kidney tissues increased in the model group (P<0.01). Compared with the model group

the blood pressure declined

the levels of mMALb

β2-MG

BUN and SCr decreased

the collagen I and collagen IV in kidney tissues decreased in the TQF group (P<0.05

P<0.01). There was no statistical difference between the TQF group and the losartan group. Conclusion: TQF taken before blood pressure peak could reduce blood pressure and decrease kidney tissue injury in SHR. The mechanism might be the synergistic effect of Yin-yang theory of inhibiting the expressions of mALb

β2-MG

SCr and collagen I

collagen IV in the kidney.