WU Liang-bing, PAN Yi, YANG Tao, et al. Effect of Tiaoganshen Qutanyu-formula on Signal Pathway of TGF--Smads of Heart and Kidney in Spontaneous Hypertensive Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 92-96.
WU Liang-bing, PAN Yi, YANG Tao, et al. Effect of Tiaoganshen Qutanyu-formula on Signal Pathway of TGF--Smads of Heart and Kidney in Spontaneous Hypertensive Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 92-96. DOI: 10.13422/j.cnki.syfjx.2015140092.
Objective: To observe the effect of Tiaoganshen Qutanyu-formula (TQF) on the pathway of transforming growth factor-β1(TGF-β1)-Smad2/3/7 in the kidney of spontaneous hypertensive rats (SHR) administered before blood pressure peak. Method: A total of 30 12-week male SHR were randomly divided into 3 groups:the TQF group (4 g · kg-1)
the losartan group (30 mg · kg-1) and the normal group. Another healthy 10 SD rats were taken as the normal group. The mRNA expressions of Smad2
Smad3 and Smad7 in the heart and kidney were detected by RT-PCR
and the protein expressions of Smad2
Smad3 and Smad7 in the kidney were determined by munohistochemical method after 12 weeks of treatment. Result: Compared with the normal group
the mRNA expressions of Smad2
Smad3 in the kidney and TGF-β1
Smad2
Smad3 in the heart increased
the mRNA expression of Smad7 in both kidney and heart decreased
the protein expressions of Smad2
Smad3 in the kidney increased
the protein expression of Smad7 decreased in the model group (P<0.01). Compared with model group
the mRNA expressions of Smad2
Smad3 in the kidney and TGF-β1
Smad2
Smad3 in the heart decreased
the mRNA expression of Smad7 in both kidney and heart increased
the protein expressions of Smad2
Smad3 in the kidney decreased in the TQF group (P<0.05
P<0.01). There was no statistical difference for protein expression of Smad7 between the TQF group and the normal group. Conclusion: TQF taken before blood pressure peak could reduce the hypertensive heart and kidney fibrosis by decreasing the aggregation of extra cellular matrix. The mechanism might be the synergistic effect of Yin-yang theory of the TGF-β1-Smads signal pathway interruption by regulating the Smad2