Objective: To compare Schisandrae Chinensis Fructus prepared with different processing methods on splenic lymphocyte proliferation in mice of. Method: The splenic lymphocyte cells were cultured with different concentrations(400

200

100

50 mg · L-1) of Schisandrae Chinensis Fructu extraction in 96-well plate

each concentration was repeated for six times. After 48 hours

the cell proliferation rate of spleen cells and T/B lymphocytes were measured by the CCK-8 colorimetric method.The T lymphocyte was induced by concanavalin A (Con A)and B lymphocyte was induced by lipopolysaccharide (LPS). Result: Compared with the blank group

Con A

LPS could induce T lymphocyte and B lymphocyte. Compared with blank group in terms of other corresponding indicators

different concentrations of Schisandrae Chinensis Fructus extraction could increase proliferation rate

especially in wine steaming Schisandrae Chinensis Fructus group with the same dosage

wine steaming Schisandrae Chinensis Fructus was better than crude Schisandrae Chinensis Fructus and vinegar steaming Schisandrae Chinensis Fructs. Conclusion: Wine steaming Schisandrae Chinensis Fructus could obviously increase the cell proliferation rate

the result is accordance with TCM theory of processed Schisandra Chinensis Fruits as Tonic.