Objective: To optimize processing technology of Bupleuri Radix with turtle blood. Method: With composite score of saikosaponin a

c

d and ethanol soluble extractives as index

an orthogonal test was adopted to investigate influence of processing time

turtle blood amount and processing temperature on processing technology of Bupleuri Radix processed with turtle blood.HPLC was adopted to determine contents of saikosaponin a

c

d and eluted in gradient with mobile phase of acetonitrile-water

detection wavelength was at 210 nm. Result: Stir-frying time had a significant influence on processing technology.According to optimum processing technology

processing temperature was set at 150 ℃

processing time was 10 min

the amount of turtle blood was 0.05 mL · g-1.Mass fractions of ethanol soluble extractives and saikosaponin a

c

d were 12.42%

0.85%

0.23%

0.90%

respectively. Conclusion: This optimized processing technology is reasonable

reliable and highly reproducible

it can provide a reference for processing of Bupleuri Radix processed with turtle blood.