Objective: Due to the difficulties of dealing with traditional Chinese medicine quality controlling

systematic quantified fingerprint method (SQFM) and the principal component analysis (PCA) were performed to evaluate the quality of Guchang Zhixie (GCZX) pill and powders. Method: The pill and powders were determined by high performance liquid chromatography with diode array detector (HPLC-DAD). Five absorption wavelengths (254

345

230

240

280 nm) were applied to reflect the characterization of six main components from GCZX pill and powders. The 12 batches analytes were processed by SQFM and PCA approaches and berberine hydrochloride was used as reference peak. Result: Refer to berberine hydrochloride peak

21 common peaks were obtained. According to the results of the SQFM and PCA analysis

qualities of all batches analytes were qualified

except the results under 230 nm. Compared to GCZX powders` fingerprint

a distinct distance from the pills could be investigated on PCA scores plot

meanwhile

9 component peaks reduced after prepared and 2 peaks disappeared around 13.05 min. Also

validation of the method has been performed. Conclusion: SQFM and PCA methods were successfully tested in GCZX pill and powders fingerprint studies. Results from assay validations showed that these two methods are selective

sensitive and robust. The developed methods were applied to Chinese traditional medicine evaluation.