Objective: Study the compatibility based on the interaction of multiple components for Sanguisorbae Radix and Sophorae Flos

and to optimize the excellent compatibility proportion. Method: Different compatibility proportion of Sanguisorbae Radix and Sophorae Flos were designed

and then extract them with hot water

the contents of total flavonoids and total tannins in each extract were determined using ultraviolet spectrophotometric method

and the DPPH radical scavenging activity was used to evaluate the antioxidant capacity

and then analyzed the relationship between DPPH radical scavenging activity and the content of total flavonoids and total tannins. Meanwhile

the HPLC fingerprints of different extracts were tested and comparative analyzed the difference between them. Result: The contents of total flavonoids and total tannins of each extracts were different

and the DPPH scavenging activity was also different. When the compatibility proportion of Sanguisorbae Radix-Sophorae Flos was 2: 1 and 1: 1

the DPPH scavenging activity was better

and the comprehensive score of the contents of total flavonoids and total tannins was the highest

which suggested that the DPPH scavenging activity was positively related to the contents of total flavones and total tannins. And the fingerprints of different extracts were different

which indicated that the compatibility proportion had some influences on chemical components. Conclusion: The contents of total flavonoids and total tannins were higher

and the DPPH scavenging activity was better than others at the compatibility proportion was 2: 1 or 1: 1 of Sanguisorbae Radix and Sophorae Flos. The experimental results can be used as a consideration of the compatibility of Sanguisorbae Radix and Sophorae Flos in clinical.