Objective: To study the effect of extracts from Taxus chinensis on cell growth and apoptosis of breast cancer cells T47D. Method: In the experiment

the blank group

different concentrations of T. chinensis extracts groups (200

100

50

25 mg · L-1) and the Adriamycin group (3 μmol · L-1) were established to treat breast cancer cells T47D for 24 h. The T47D cell proliferation was determined by MTT method. The activity of lactate dehydrogenase (LDH) and Caspase-3 were detected by kit. The cell apoptosis was tested by Hoechst33342 fluorescence. The expressions of proteins related to T47D cell apoptosis

such as pro-apoptosis proteins(Bax)

anti-apoptotic proteins (Bcl-2) and Caspase-3

were detected by Western blot method. Result: Compared with the blank group

extracts from T. chinensis significantly inhibited the proliferation of T47D cells

improved the LDH and Caspase-3 activities

induced the apoptosis of T47D

up-regulated the level of Bax and down-regulated the level of Bcl-2

with significantly statistical differences (P<0.05

P<0.01) and concentration dependence. Conclusion: Extracts from T. chinensis have a direct inhibitory effect on breast cancer cells T47D proliferation

with concentration dependence. Its mechanism may be related to the induction of cell apoptosis.