Objective: To study the antioxidant activity of cyclophosphamide (CTX) combined with ethyl acetate extracts from persimmon leaves (PE) on hepatoma H22 bearing mice and their effect on tumor tissue apoptosis-related proteins Bcl-2 and Bax expressions. Method: Kunming mice were selected and subcutaneously inoculated with H22 tumor at right fore armpit to establish the H22 bearing mouse model. The H22 bearing mice were randomly divided into the model group

the CTX group (0.1 g · kg-1)

the CTX combined with high

middle and low dose PE groups (2

1

0.5 g · kg-1) and were orally given drugs for 10 days. After killing the rats

efforts were made to calculate the anti-tumor rate

detect the combined effect by Jin's formula q

superoxide dismutase(SOD) activity and malonaladehyde(MDA) content in tumor tissues

and analyze Bcl-2 and Bax protein expressions in tumor tissues by immunohistochemistry. Result: Compared with the model group

all treatment groups showed significantly higher tumor inhibition rate

SOD and Bax protein expression and lower MDA and Bcl-2 protein expression (P<0.05

P<0.01). The tumor inhibition rate of CTX combined with PE in each dose group was higher than that of the CTX group

with the Jin's formula q between 0.85 and 1.15

indicating their combined effect. Compared with the CTX group

the SOD activity and bax expression of CTX combined with PE in each dose group increased

but the MAD content

Bcl-2 expression and Bcl-2/Bax ratio decreased (P<0.05

P<0.01). Conclusion: CTX combined with PE had better antitumor effect. Its mechanism is probably related to their combined effect in notably strengthening the antioxidant capacity

down-regulating Bcl-2 expression and up-regulating bax expression of H22 bearing mice.