Objective: To study the clinical efficacy of Wenyang Jianpi decoction in treating spleen-kidney yang deficiency type laxative constipation and stem cell factor(SCF)/c-kit signal pathway. Method: The eighty patients with spleen-kidney Yang deficiency type laxative constipation treated in our hospital during January 2013 to September 2014 were randomly divided into the control group (mosapride) and the observation group (Wenyang Jianpi decoction)

with 40 patients in each group. After a 4-week treatment

the changes in clinical efficacy

traditional Chinese medicine(TCM) symptom scores before

during and after the treatment were observed;and the protein expressions of colon c-kit and SCF were detected by western blot. Result: Compared with the control group

the total effective rate significantly increased in the observation group

with statistical difference (90.0%VS 70.0%

P<0.05);compared with the tyrosine kinase receptor control group

the TCM symptom scores after the treatment obviously decreased in the observation group

with statistical difference (4.8±2.3 VS 8.2±3.4

P<0.05);compared with the pre-treatment groups

the protein expressions of colon tyrosine kinase receptor (c-kit) and stem cell factor (SCF) in the post-treatment groups significantly increased

with statistical difference (P<0.05);compared with the control group

the protein expression of colon c-kit significantly increased in the post-treatment observation group

with statistical difference (0.39±0.17 VS 0.28±0.15

P<0.05) and the protein expression of colon SCF showed no significant changes and statistical difference (0.72±0.23 VS 0.71±0.20). Conclusion: The Wenyang Jianpi decoction can significantly increase the protein expression of colon c-kit and SCF and relieve the clinical symptoms of patients with spleen-kidney Yang deficiency type laxative constipation through SCF/c-kit signal pathway.