Objective: To investigate urine metabolic profile of rats with deficiency-cold hemorrhagic disease

and discuss intervention effects of dried and charred Zingiberis Rhizoma Recens. Method: Proton nuclear magnetic resonance (1H-NMR) technique was used to analyze metabolome in urine.Partial least squares discriminant analysis (PLS-DA) was processed to analyze metabonome difference among four groups.Seven potential biomarkers were selected by variable importance in the projection (VIP).Principal component analysis (PCA) was employed to process data from the charred Zingiberis Rhizoma Recens treatment group

hemostatic effects of charred Zingiberis Rhizoma Recens were investigated through the content of potential biomarkers. Result: Charred Zingiberis Rhizoma Recens could make disorder endogenous biomarkers of the model rats to normal levels

while dried Zingiberis Rhizoma Recens had weaker efficacy.Acetoacetic acid

pyruvic acid

nitrogen oxygen trimethylamine

taurine and tryptophan were efficacy of hemostasis biomarkers in charred Zingiberis Rhizoma Recens. Conclusion: Metabolomics can reflect the whole status of metabolism in biosystems

it can be treated as a potential powerful approach in pharmacological studies for different processed products and investigation of essence of syndrome in traditional Chinese medicine.