Objective: To study the effect of representative Qi-tonifying and blood-activating formulae Buyang Huanwu Tang (BYHWT) on the atherosclerosis model

in order to observe the aorta Rho kinase

PAl-1 and eNOS mRNA expressions and blood lipid indicator and reveal new targets and new ways of the formulae in inhibiting atherosclerosis. Method: Totally 60 rats were randomly divided into six groups:the normal control group

the model group

BYHWT low and high dose of groups (10

20 g · kg-1)

the Simvastatin group (0.6 mg · kg-1) and the BYHWT prevention group (10 g · kg-1). Except for the normal group

the other groups were fed with VD3 and high fat diet to induce atherosclerosis and given corresponding drugs. The BYHWT prevention group was administered with drugs during the modeling. After the successful modeling and 28-day intervention

the expression level of Rho kinase

plasma tissue plasminogen activator inhibitor 1(PAl-1)

endothelial nitric oxide synthase(eNOS) mRNA expression

total cholesterol(TC)

triglyceride(TG)

high density lipoprotein cholesterin(HDL-C)

low density lipoprotein cholesterin(LDL-C) and oxidized low-density lipoprotein(ox-LDL) in atherosclerosis aorta were detected. Result: Compared with the normal group

the model group showed significantly higher Rho kinase

PAl-1 mRNA expression

liquid TC

TG

HDL-C

ox-LDL and lower eNOS mRNA expression and LDL-C

all with significant statistical differences (P <0.01);BYHWT low and high dose groups

the Simvastatin group and the BYHWT prevention group showed notably lower Rho kinase

PAl-1 mRNA expression

lipid TC

TG

HDL-C

ox-LDL and higher eNOS mRNA expression

LDL-C

with significant statistical differences (P <0.05

P <0.01). Conclusion: BYHWT has an effect in down-regulating the Rho kinase and PAl-1 mRNA expression

up-regulating the eNOS mRNA expression and blood lipid

resisting atherosclerosis

inhibiting the Rho kinase and PAl-1 mRNA expression and increasing the Rho kinase and eNOS mRNA expression

which may be related to its mechanisms.