Objective: To explore the protective effect of Lini Semen water extract and alcohol extract on D-GaIN induced acute liver injury in mice. Method: The mice acute liver injury model was induced by D-GaIN.90 mice were divided into 9 groups

blank group

model group

Bifendate pills group

Lini Semen water extract and alcohol extracts

low

middle

high dose group. The blank group and the model group were given volume saline

the other groups were given corresponding drugs dose by gavage once daily for 10 d.At the ninth day of administration

according to the dosage of 600 mg · kg-1 intraperitoneal injection of 10% D-GaIN amine solution was given toestablish the modle. 24 h after the last administration blood samples were collected to measure serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity.Malondialdehyde(MDA)

glutathione(GSH)

superoxide dismutase(SOD) in liver tissue and liver index were measured. The hepatic histological changes were observed by HE with light microscope. Result: Compared with the normal group

model group significantly increased ALT

AST

MDA levels (P <0.05

P <0.01)

decreased SOD and GSH activity (P <0.01)

hepatic tissue structure was not clear

dissolved necrosis of liver cells were obviously;observed.Compared with the model group

the Lini Semen water extraction high dose and alcohol extraction low dose groups could increase the activity of serum SOD (P <0.05)

decrease the level of MDA (P <0.01). Lini Semen treatment group decrease serum ALT

AST and the liver index

and improved hepatic tissue structure

reduced the number of necrosis of liver cells

liver cell regeneration was observed.The high dose group improved obviously. Conclusion: Lini Semen extract has certain protective effect on acute liver injury induced by D-GaIN in mice.