In order to strengthen the research and development of Cordyceps nucleosides

multiple detection methods adopted for Cordyceps nucleosides at home and abroad were reviewed. Three detection technologies used in the analysis of Cordyceps nucleoside were selected

including high performance liquid chromatography (HPLC)

capillary electrophoresis (CE) and thin layer chromatography scanning (TLCs) method. According to different detectors

HPLC was also classified and compared. The HPLC method is usually applied in the qualitative and quantitative analysis of nucleosides

with a high sensitivity

quick separation and high separation efficiency. Among them

the LC-MS method features a wider adaptability

strong separation capacity and low detection limit and can provide rich structure and molecular weight information of each component. The LC-MS method has been widely used not only in the detection of Cordyceps nucleosides

but also in intensive studies on Cordyceps nucleosides or other drugs containing nucleosides at the molecular level.