Objective: To establish an HPLC multi-wavelength method for determination of mangiferin

paeoniflorin

prim-O-glucosylcimifugin

iquiritin

5-O-methylvisamminol

isoliquiritoside and glycyrrhizic acid in Guizhi Shaoyao Zhimu Tang. Method: Zorbax SB-C18 column (4.6 mm×250 mm

5 μm) was eluted with the mobile phase of acetonitrile-0.01% formic acid water in a gradient mode. The flow rate was 1 mL·min-1.The detection wavelength was set at 250 nm (mangiferin)

237 nm (paeoniflorin

prim-O-glucosylcimifugin

glycyrrhizic acid)

280 nm (iquiritin

4'-O-β-glucopyranosyl-5-O-methylvisamminol)

and 355 nm (isoliquiritoside). The column temperature was maintained at 30 ℃. Result: Mangiferin

paeoniflorin

prim-O-glucosylcimifugin

iquiritin

5-O-methylvisamminol

isoliquiritoside and glycyrrhizic acid had a good linearity within the ranges of 0.264 8-2.648(r=0.999 9)

0.81-8.10(r=0.999 8)

0.230 4-2.304(r=0.999 9)

0.173-1.73(r=0.999 9)

0.115-1.15(r=0.999 8)

0.028 4-0.284(r=0.999 8)

0.252 4-2.524 μg(r=0.999 9)

with the recoveries between 97.41%-102.0% and RSD<3.0%. Conclusion: This method is simple

accurate and highly reproducible and can provide basis for studying material base of Guizhi Shaoyao Zhimu Tang.