Objective: To investigate effect of vinegar-baked Bupleuri Radix on distribution of gentiopicroside in mice

and discuss liver induced mechanism of vinegar-baked Bupleuri Radix. Method: Male mice were divided into four groups

including gentiopicroside control and gentiopicroside coadministered with three different doses of vinegar-baked Bupleuri Radix.Samples were collected at time points of 0.08

0.25

0.5

0.75

1

1.5

2

4 h

6 mice were used in every time point.Samples were detected by HPLC-MS/MS.Targeting effect of different tissues of vinegar-baked Bupleuri Radix on gentiopicroside was evaluated through parameters of relative tissue exposure (Re) and relative targeting efficiency (RTE). Result: Three different doses of vinegar-baked Bupleuri Radix could increase RTE of gentiopicroside in liver.RTE values of the low

medium and high dose groups in liver were 0.25

0.13 and 0.33.Although uptake of gentiopicroside in liver was not increased

vinegar-baked Bupleuri Radix could decrease distribution of gentiopicroside in other tissues except liver significantly. Conclusion: Vinegar-baked Bupleuri Radix

the meridian guide drug

can increase RTE of liver by reducing distribution of gentiopicroside in other tissues except liver.