Objective: Geniposidic acid (GPA) can promote bile acid excretion

its metabolism process difference was studied in cholestatic model for development clinical role. Method: Rats were divided into the normal group and the cholestatic model group induced by α-naphthyl isothiocyanate (ANIT

dose of 100 mg·kg-1)

different doses of GPA were administered (high

medium and low doses of 100

50

25 mg·kg-1).GPA and chlorogenic acid were extracted from plasma by protein precipitation

separated on phenomenex C18 column with a mobile phase of acetonitrile and water containing 0.5% acetic acid with rate of (28: 72)

detection wavelength was at 238 nm.Stata12.0 was adopted to fit compartment model and calculate pharmacokinetic parameters. Result: ANIT cholestasis model made a success rate of more than 95%.Compared with the normal group

rat with cholestasis model might reduce Ke and increase T1/2 of GPA in vivo

and might extend the mean residence time (MRT0-t) in vivo

also might improve AUC0-t and AUC0-∞. Conclusion: This analytical method is suitable and reliable for in vivo analysis of GPA

cholestasis model can reduce elimination and excretion of GPA.