Objective: To study the effect of Yishen Daluo decoction on phosphated c-Jun N-terminal kinase 1 and 2 (p-JNK1/2)

c-Jun N-terminal kinase 1 and 2 (JNK1/2) and cyclooxygenase 2 (COX-2) in mice with experimental autoimmune encephalomyelitis (EAE). Method: Totally 48 female SJL mice (aged 8-12 weeks) were randomly divided into 4 groups

the normal group

the model group

the Chinese medicine (CM) group and the Western medicine (WM) group

with 12 mice in each group. Each mouse in model

CM and WM groups was injected subcutaneously with 200 μL antigen emulsion [including 150 μg proteolipid protein(PLP)139-151 and 400 μg H37 Ra] at two sites of epigastrium on the first day

100 μL Bordetella pertussis liquid (containing 6×105 B.pertussis) was injected to each mouse by caudal vein at the 1st and 3rd days. At the 7th day

10 mL·kg-1 of saline was orally given to each mouse in normal and model groups

20 g·kg-1of crude Yishen Daluo decoction was orally given to the CM group

and 3.9 mg·kg-1 of prednisone was orally given to the WM group for 15 days. After the immunization

the neurological score for each group was recorded every day. Western blot was used to detect the expressions of p-JNK1/2

JNK1/2 and COX-2 in cerebral tissues of EAE mice. Result: The neurologicalscore significantly increased in EAE mice of the model group compared with the model group (P<0.01). The expressions of p-JNK1/2 and COX-2 in the model group increased (P<0.01). Compared with the model group

CM and WM groups showed obvious decreases in the neurological score (P<0.05) and expressions of p-JNK1/2

JNK1/2 (P<0.05

P<0.01)

and the WM group showed a lower COX-2 expression than the CM group (P<0.05). Conclusion: The JNK1/2 pathway and COX-2 are correlated with the onset of EAE. Yishen Daluo decoction can reduce the neurological score of EAE by decreasing the expressions p-JNK2 and COX-2.However

further studies shall be conducted for its specific mechanism.