Objective: To compare contents of five main effective components (aucubin

geniposidic acid

chlorogenic acid

catechin and rutin) in Eucommiae Folium from 17 different areas. Method: HPLC was employed to determine contents of five main effective components with mobile phase of acetonitrile-0.1% phosphoric acid for gradient elution and detection wavelength at 208 nm.Contents of five main effective components in Eucommiae Folium from 17 different areas were investigated. Result: There were significant differences between contents and total content of these five main effective components in Eucommiae Folium from different areas.The highest total content of five effective components was 14.15% which was from Huangling county

Shaanxi province;samples with the lowest total content (1.37%) of them was from Quxian county

Sichuan province;difference of these two values was more than 10-fold. Conclusion: There are some regional differences of contents of five main effective components in Eucommiae Folium. The total content of main effective components in samples from Shaanxi and Henan province is more than from southwest area of our country

such as Sichuan

Guizhou province and Chongqing city.Eucommiae Folium should be possible to take low temperature rapid drying.