Objective: To develop a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous determination of five components (gastrodin

militarine

loroglossin

arbutin

4-hydroxybenzenemethanol) in Gymnadeniae Conpseae Rhizoma

in order to provide basis for the quality control. Method: Gastrodin was used as the internal reference substance

and the relative correlation factors (RCF) with militarine

loroglossin

arbutin and 4-hydroxybenzenemethanol were determined by HPLC-UV. The contents of the four components were calculated according to the RCF

respectively. The contents of these components in 10 batches of Gymnadeniae Conpseae Rhizoma were determined by the external standard method. The rationality

feasibility

and repeatability of the QAMS method were verified by comparing the results obtained from the two different methods. Result: RCF of gastrodin-arbutin

gastrodin-4-hydroxybenzenemethanol

gastrodin-militarine and gastrodinloroglossin were 3.083 1(2.0%)

1.562 2(2.1%)

1.545 0(1.7%)

1.742 5(1.9%)

respectively. For the five components

there was no significant difference between the calculated and determined values RSD(<3%)of the 10 batches by the two different methods. Conclusion: The method established in this study is accurate and feasible and can be used for the determination of five components in Gymnadeniae Conpseae Rhizoma simultaneously.