柱前衍生RP-HPLC测定仙灵骨葆胶囊中16种氨基酸

杨卫灵; 李朝婵; 田红红; 谭高好; 柳立伟; 伍庆

doi:10.13422/j.cnki.syfjx.2015180044

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柱前衍生RP-HPLC测定仙灵骨葆胶囊中16种氨基酸

化学与分析 | 更新时间：2024-01-04

- 柱前衍生RP-HPLC测定仙灵骨葆胶囊中16种氨基酸
- Determination of 16 Amino Acids in Xianling Gubao Capsule by RP-HPLC with Pre-column Derivatization
- 中国实验方剂学杂志 2015年21卷第18期页码：44-47
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技支撑计划项目(2011BAI13B04-01)
- DOI：10.13422/j.cnki.syfjx.2015180044
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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杨卫灵, 李朝婵, 田红红, 等. 柱前衍生RP-HPLC测定仙灵骨葆胶囊中16种氨基酸[J]. 中国实验方剂学杂志, 2015,21(18):44-47.

YANG Wei-ling, LI Chao-chan, TIAN Hong-hong, et al. Determination of 16 Amino Acids in Xianling Gubao Capsule by RP-HPLC with Pre-column Derivatization[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(18): 44-47.
杨卫灵, 李朝婵, 田红红, 等. 柱前衍生RP-HPLC测定仙灵骨葆胶囊中16种氨基酸[J]. 中国实验方剂学杂志, 2015,21(18):44-47. DOI： 10.13422/j.cnki.syfjx.2015180044.

YANG Wei-ling, LI Chao-chan, TIAN Hong-hong, et al. Determination of 16 Amino Acids in Xianling Gubao Capsule by RP-HPLC with Pre-column Derivatization[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(18): 44-47. DOI： 10.13422/j.cnki.syfjx.2015180044.

摘要

目的: 建立柱前衍生RP-HPLC同时测定仙灵骨葆胶囊中16种氨基酸的方法。方法: 以正亮氨酸为内标物

异硫氰酸苯酯(PITC)为柱前衍生剂

用高效液相色谱测定仙灵骨葆胶囊中水解氨基酸的量。采用Hypersil C18色谱柱(4.6 mm×250 mm

5 μm)

以乙腈-0.1 mol·L-1乙酸钠溶液-乙酸(7:93:0.05)为流动相A

乙腈-水(80:20)为流动相B进行梯度洗脱

体积流量1 mL·min-1

检测波长254 nm

柱温40 ℃。结果: 33 min内可完成仙灵骨葆胶囊中16种氨基酸的分离测定

线性关系良好(r>0.996 8)

回收率97.5%~100.7%

RSD<5%。结论: 该方法简便快速、准确可靠

可用于仙灵骨葆胶囊中氨基酸的含量测定。

Abstract

Objective: To develop a RP-HPLC method for the simultaneous determination of 16 amino acids in Xianling Gubao capsule. Method: With norleucine as the internal standard

hydrolyzed amino acids of Xianling Gubao capsule were derivatized with phenylisothiocyanate (PITC) and determined by RP-HPLC. Hypersil C18 column (4.6 mm×250 mm

5 μm) was eluted with mobile phase A acetonitrile-0.1 mol· L-1 sodium acetate solution-acetic acid (7:93:0.05) and mobile phase B acetonitrile-water (80:20) in a gradient mode at a flow rate of 1 mL· min-1. The detection wavelength was set at 254 nm and the column temperature was 40 ℃. Result: The 16 amino acids were separated within 33 min with a good linearity (r>0.996 8) within the range of the test concentration. The average recoveries ranged between 97.5% and 100.7% and the RSD values were less than 5%. Conclusion: The method is simple

quick

accurate and reliable and can be used for the determination of amino acids in Xianling Gubao capsule.

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