Objective: To optimize HPLC methods of loganin

paeonol and verbascoside

we detected the content of three components in Liuwei Dihuang Wan that prepared by sulfur-fumigated Dioscoreae Rhizoma

in order to reveal the effect of sulfur-fumigation on Traditional Chinese Medicine formulae. Method: The column was a Thermo Hypersil BDS C18(4.6 mm×250 mm

5 μm). Loganin was gradiently eluted by acetonitrile-0.1% phosphoric acid with water system as mobile phase

and 236 nm as the detection wavelength. Paeonol was isocraticly eluted by acetonitrile-0.1% phosphoric acid with water (50:50)

and 274 nm as the wavelength. Verbascoside was gradiently eluted by acetonitrile-0.1% phosphoric acid with water system as mobile phase

and 236 nm as the wavelength. The column temperature was 30 ℃

the flow rate was 1 mL· min-1

the injection volume was 10 μL. Result: With the increase of SO2 amount

loganin

paeonol and verbascoside derived from Liuwei Dihuang Wan were obviously decreased. In thesodium sulfite group

the content of the three components was the lowest

which to 0.022 6

0.691 2 and 0.036 2 mg· g-1. Conclusion: The optimized HPLC methods had good accuracy

sensitivity and repeatability

which was suitable for determining in Liuwei Dihuang Wan. The results indicated that sulfur-fumigated Dioscoreae Rhizoma would have an adverse effect on Liuwei Dihuang Wan;the possible reason came from SO2 residue.