Objective: To study the Chemical Constituents of Uncariae Ramulus Cum Uncis. Method: The compounds were obtained and purified by column chromatography on silica gel column

Sephadex LH-20

ODS and preparative HPLC

the structures of these compounds were identified according to their physicochemical properties and spectral data. Result: Fourteen compounds were obtained from the ethanol extract of Uncariae Ramulus Cum Uncis

their structures were elucidated as β-sitosterol (1)

daucosterol (2)

chlorogenicacid (3)

corynoxine (4)

corynoxeine (5)

isocorynoxeine (6)

rhynchophylline (7)

isorhynchophylline (8)

protopine (9)

cadambine (10)

3α-dihydrocadambine (11)

vincoside lactam (12)

strictosamide (13)

hyperoside (14). Conclusion: Compound 9 was obtained from this genus for the first time. Compounds 3

13 and 14 were obtained from the plant for the first time.