Objective: To develop a HPLC method of determination of 1-deoxynojirimycin in Jintangning capsules. Method: The samples were collected after elution with ammonia by processed D001-CC type positive cation exchange resin and pre-column derivatization with the concentration of 2 g· L-1 of 9-fluorenylmethyl chloroformate (Fmoc-Cl) and then determinated by DAD detector. ODS-C18(4.0 mm×250 mm

5 μm)was eluted with the mobile phase of acetonitrile and 0.2% phosphoric acid in a gradient mode. The flow rate was 1.0 mL· min-1

the detection wavelength was 263 nm

and column temperature at 25 ℃. Result: 1-deoxynojirimycin had good linear relationships within the range of 0.84-8.4 μg (r=1.000 0)

with the precision RSD of 0.3% and repetition RSD of 0.6%

the sample solution after derivatization was stable within 24 hours. The average recovery was 109.6%

and the relative standard deviations were 0.4%. Conclusion: The method is stable

reliable and accurate

and can be used for the determination of 1-deoxynojirimycin in Jintangning capsules.