Objective: To detect serum metabolites in rats with chronic myocardial ischemia after intragastric administration of Guizhi decoction. Method: Rats were divided into 3 groups randomly

included the Guizhi group

the model group and the normal group.The normal group was given normal saline with dose of 0.04 mg· kg-1 once a day simultaneously

the other groups were intraperitoneal given isoprenaline hydrochloride with dose of 0.04 mg· kg-1 once a day

six days in a row

after modeling

the Guizhi group was intragastric administration of Guizhi decoction with dose of 0.625 g· mL-1

then after two weeks

HE staining of the heart tissue of rats was observed.Characteristics of serum metabolites in rats were measured by nuclear magnetic resonance mass spectrometry

principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Result: Compared with the model group

rat cells of the Guizhi group was completed with neat muscle fiber and no edema.Rat serum samples of the Guizhi group were closely to the normal group

two samples were separated with model accurate parameter of Q2>0.9.Twenty-eight metabolites were detected in rat serum of three groups

including carbohydrate

amino acids

organic acids and so on. Conclusion: Guizhi decoction can reduce the degree of chronic myocardial injury.Metabonomics is an effective technique for detecting traditional Chinese medicine in the treatment of cardiovascular disease.