Objective: To compare effect of different compatibility of Platycodonis Radix and Achyranthis Bidentatae Radix on tissue distribution of paeoniflorin in normal and blood stasis mice

preliminary study leading action of Platycodonis Radix and Achyranthis Bidentatae Radix. Method: Normal and blood stasis mice were gavaged with Xuefu Zhuyu Tang

lacked Platycodonis Radix or Achyranthis Bidentatae Radix parties.Mice were killed after 30 minutes

whole blood collected

the brain

heart

liver

spleen

lung and kidney were removed.HPLC was employed to determine the content of paeoniflorin with mobile phase of acetonitrile-water (16:84)

detection wavelength at 230 nm and geniposide as internal standard. Result: After normal mice being gavaged with Xuefu Zhuyu Tang full party

single lacked Platycodonis Radix party

single lacked Achyranthis Bidentatae Radix party

lacked Platycodonis Radix and Achyranthis Bidentatae Radix party

the content of paeoniflorin in lung were 0.010 2

0.005 8

0.091 0

0.037 3 μg· g-1

those in kidney were 0.122 2

0.052 0

0.144 0

0.065 0 μg· g-1

but paeoniflorin were unchecked in the brain

heart

liver and spleen.After blood stasis mice being gavaged with Xuefu Zhuyu Tang full party

single lacked Platycodonis Radix party

the content of paeoniflorin in lung were 0.076 4

0.042 0 μg· g-1

those in kidney were 0.335

0.210 μg· g-1

but it was unchecked in the brain

heart

liver and spleen.After blood stasis mice being gavaged with Xuefu Zhuyu Tang single lacked Achyranthis Bidentatae Radix party

lacked Platycodonis Radix and Achyranthis Bidentatae Radix party

the content of paeoniflorin in lung were 0.274 7

0.019 7 μg· g-1

those in kidney were 0.467 1

0.149 0 μg· g-1

it almost were unchecked in the brain

heart

liver and spleen. Conclusion: Making comparison based on Xuefu Zhuyu Tang lacked Platycodonis Radix and Achyranthis Bidentatae Radix party

Platycodonis Radix can increase the content of paeoniflorin in the lung and kidney in normal mice

Platycodonis Radix can more significantly increase the content of paeoniflorin in the lung than that in the kidney in blood stasis mice.Achyranthis Bidentatae Radix can decrease the content of paeoniflorin in the lung and kidney in normal mice

it can increase the content of paeoniflorin in the lung and kidney in blood stasis mice

it also can make distribution of paeoniflorin in the heart

liver

spleen in blood stasis mice.This study confirms Platycodonis Radix and Achyranthis Bidentatae Radix have effect of leading action.Leading sites of Platycodonis Radix and Achyranthis Bidentatae Radix are not single.