Objective: To investigate protective effect of polysaccharide from Vitis viniferal (VTP) on expression of beta-amyloid precursor protein (APP) in PC12 cells induced by β-amyloid protein25-35(Aβ25-35)

in order to discuss the mechanism of VTP in pathogenesis of Alzheimer's disease (AD). Method: Alzheimer's disease (AD) cell model was established by inducing PC12 cells with 20 μmol· L-1 Aβ25-35 for 24 h and intervened with different concentrations of VTP (20

40

80 mg· L-1). Cell viability was detected by CCK-8.The activities of dismutase superoxide(SOD) and malondialdehyde(MDA) were measured. The expression of APP mRNA was quantified by real-time PCR. Result: Compared with the normal group

the model group showed decreases in cell viability and SOD activity (P<0.01) and increases in MDA content and APP mRNA expression (P<0.01). Compared with the model group

cell viability and SOD activity were increased (P<0.05)

MDA content and APP mRNA expression were decreased in VTP groups (P<0.05)

indicating the relief in neuronal injury. Conclusion: VTP has a protective effect in the oxidative injury induced by Aβ25-35 and plays a role in preventing and treating AD by resisting oxidation

adjusting APP mRNA expression

inhibiting Aβ formation and proteting neurocytes.