Objective: To study the effect of Kangxianling docotion on PI3K/AKT/mTOR signaling pathway and its mechanism against renal fibrosis. Method: Totally 60 C57 mice were randomly divided into sham group (n=10) and operation group (n=50)

the surgical group received 5/6 nephrectomy. After two weeks

the surgery group were randomly divided into model group

Kangxianling low

medium and high dose groups and rapamycin positive control group

with 10 in each group. The sham group received 0.5 mL of normal saline

ig

Kangxianling docotion low

medium and high dose groups were given 0.5 mL Kangxianling drug orally (0.1

0.2

0.4 mg· kg-1)

rapamycin group was given 0.5 mL rapamycin gavage (0.016 μg· kg-1). Mice were sacrificed after 12 weeks of gavage. The 24 h urine protein before the mice were sacrificed to detect 24 h protein quantification

the orbital blood was collected to test serum creatinine

blood urea nitrogen;the remnant kidney was collected to observe renal morphology by HE change;PCR was used to detect PI3K/AKT/mTOR mRNA expressions in kidney tissues. Result: Compared with sham operation group

model group showed significantly higher 24-hour urinary protein excretion

serum creatinine

blood urea nitrogen

PI3K/AKT/mTOR mRNA expressions (P<0.01)

with significantly renal pathological changes. Compared with the model group

all treatment groups showed lower 24-hour urinary protein excretion

serum creatinine

blood urea nitrogen

PI3K/AKT/mTOR mRNA expressions (P<0.05)

with improvements in renal histology. Conclusion: Kangxianling can decrease 24 h urinary protein quantification in mice

improve renal function and delay the occurrence of renal fibrosis;its mechanism may be related to the inhibition of the expression pathway PI3K/AKT/mTOR signal.