Objective: To observe the clinical effect of modified Buzhong Yiqi Tang in treating recurrent spontaneous abortion (spleen kidney deficiency type) caused by negative closed antibody and its effect on levels of serum interleukin-6 (IL-6)

tumor necrosis factor-α (TNF-α)

γ-interferon (IFN-γ)

interleukin-10 (IL-10). Method: Seventy-three patients were randomly divided into the experimental group (38 cases) and control group (35 cases) according to the number table method. For control group

patients took spouse in vitro lymphocyte-induce immune therapy. In addition to the therapy of control group

experimental group was given Buzhong Yiqi Tang from the 3rd month before preganecy to the 16 weeks of gestation. For the two groups

they were all followed up until delivery. Conception

the number of weeks exceeding the original gestational weeks

gestational age up to 12 weeks and term birth were recorded. The spleen kidney deficiency syndrome before and after treatment were scored. The conversion of three negative closed antibody (anti-CD3-BE

anti-CD4-BE and anti-CD8-BE) to positive were detected. The levels of serum IL-6

TNF-α

IFN-γ and IL-10 before and after treatment were tested. Result: There was no statistical significance in difference between pregnancy rate and the number of weeks exceeding the original gestational weeks in two groups. The gestational age up to 12 weeks and the rate of term birth in experimental group were superior to these data in control group (P<0.05). After treatment

positive conversion rate of anti-CD3-BE and anti-CD8-BE in experimental group were higher than these data in control group (P<0.05). Levels of TNF-α and IFN-γ in experimental group were superior to these in control group

while the IL-6 and IL-10 were higher than the standards in control group (P<0.01). The total effective rate was 89.47% in experimental group

which was better than 71.43% in control group (P<0.05). Conclusion: Modified Buzhong Yiqi Tang combined with in vitro spouse lymphocyte-induce immune therapy can treat recurrent spontaneous abortion caused by negative closed antibody

promote the positive conversion of closed antibodies

enhance the rate of childbirth pregnancy and relieve clinical symptoms. It has distinct clinical curative effect.