Objective: To optimize purification process of total flavonoids from four Crataegus fruits in Xinjiang by macroporous resin and evaluate its antioxidant activity. Method: With purity of total flavonoids as index

13 macroporous resins for adsorption and separation of total flavonoids were compared.Purification process of total flavonoids was optimized by screening flow velocity of sample

concentration of eluent.Antioxidant activity was determined by 2

2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS) and 2

2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method.Contents of rutin and hyperin were determined by HPLC with mobile phase of acetonitrile-0.4% formic acid (16:84) and detection wavelength at 360 nm. Result: HPD722 resin offered the best adsorption and desorption capacity for total flavonoids.Optimal purification process was as follows:flow velocity of sample 0.84 mL·min-1

after reaching adsorption balance

dynamically eluted with 40% ethanol.After separated and purified by HPD722 resin

contents of total flavonoids

rutin

hyperin and antioxidant capacity increased 1.9-3.3

2.4-5.1

2.6-3.7

2.0-4.6 times

respectively. Conclusion: Total flavonoids from four C. fruits can be effectively purified and enriched by HPD-722 macroporous resin.