Objective: To choose the best DNA extraction method for different kind of Tupistra chinensis materials. Method: Sodium dodecyl sulfate (SDS)

improved SDS

hexadecyl trimethyl ammonium bromide (CTAB) and improved CTAB methods were used to extract DNA from five different materials of T. chinensis tubers and leaves

including fresh tubers

silica gel dried tubers

baked tubers

fresh leaves and silica gel dried leaves.Integrity

purity and yield of extracted DNA was compared in order to determine which method was the best one for different T. chinensis materials. Result: Extracting DNA from silica gel dried tubers and silica gel dried leaves with improved CTAB method performed the highest purity and good integrity

not only its value of A260 nm/A280 nm was between 1.8-1.9

but also with a better integrity.These remaining materials had different levels of pollution

A260 nm/A280 nm of fresh leaves was generally low. Conclusion: Improved CTAB is the most effective method for DNA extraction from silica gel dried leaves and tubers materials.Genomic DNA extracted from silica gel dried leaves and tubers using improved CTAB method performs a better integrity and better purity.