Objective: To prepare curcumin-quinacrine liposomes

then optimize its formulation technology and evaluate its quality in order to provide references for development of curcumin. Method: Curcumin-quinacrine liposomes were prepared by film dispersion-ammonium sulfate gradient method.The content of curcumin and quinacrine were determined by HPLC

mobile phase was methanol-water (containing 0.3% acetic acid) (70:30) and acetonitrile-water (containing 0.6% acetic acid and 1.5% triethylamine) (50:50)

flow velocity was 1.0

1.5 mL·min-1

detection wavelength was 423

269 nm

respectively.Formulation process of curcumin-quinacrine liposomes was optimized by single factor tests with encapsulation efficiency as index.Characterization of curcumin-quinacrine liposomes were investigated

including particle size and Zeta potential. Result: Parameters of optimal prescription were mole ratio of phospholipid-cholesterol (2:1)

curcumin-membrane material (1:40)

quinacrine-membrane material (1:48) and ammonium sulfate concentration of 250 mmol·L-1.Encapsulation efficiencies were (95.33±0.85)% and (94.13±0.49)% for curcumin and quinacrine

respectively.Particle size was (117.73±0.15) nm

Zeta potential was (-8.56±0.22) mV

polydispersity index (PDI) was 0.12±0.01. Conclusion: Curcumin-quinacrine liposomes has advantages of uniform particle size

high encapsulation efficiency

good stability and enough slow-release effect.