UPLC-MS/MS同时测定双黄连片中5种有效成分的含量

殷爱玲; 谈瑄忠

doi:10.13422/j.cnki.syfjx.2015190057

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UPLC-MS/MS同时测定双黄连片中5种有效成分的含量

化学与分析 | 更新时间：2024-01-04

- UPLC-MS/MS同时测定双黄连片中5种有效成分的含量
- Simultaneous Determination of Five Effective Components in Shuanghuanglian Tablet by UPLC-MS/MS
- 中国实验方剂学杂志 2015年21卷第19期页码：57-59
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- DOI：10.13422/j.cnki.syfjx.2015190057
  中图分类号： O657.63;R286.0
- 纸质出版日期：2015
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殷爱玲, 谈瑄忠. UPLC-MS/MS同时测定双黄连片中5种有效成分的含量[J]. 中国实验方剂学杂志, 2015,21(19):57-59.

YIN Ai-ling, TAN Xuan-zhong. Simultaneous Determination of Five Effective Components in Shuanghuanglian Tablet by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(19): 57-59.
殷爱玲, 谈瑄忠. UPLC-MS/MS同时测定双黄连片中5种有效成分的含量[J]. 中国实验方剂学杂志, 2015,21(19):57-59. DOI： 10.13422/j.cnki.syfjx.2015190057.

YIN Ai-ling, TAN Xuan-zhong. Simultaneous Determination of Five Effective Components in Shuanghuanglian Tablet by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(19): 57-59. DOI： 10.13422/j.cnki.syfjx.2015190057.

摘要

目的:建立同时测定双黄连片中5种有效成分含量的UPLC-MS/MS分析方法。方法:采用BEHC18色谱柱(2.1 mm×100 mm

1.7μm)

流动相乙腈-0.1%甲酸水梯度洗脱

流速0.4 mL·min-1

采用电喷雾电离源(ESI)

多反应离子监测(MRM) 扫描方式。结果:10种被测物呈良好的线性关系(r>0.999);精密度、重复性和稳定性良好;加样回收率在96.00%~104.67%

RSD均<3%。结论:所建立的方法准确、快捷

重复性好

可用于双黄连片中绿原酸、连翘苷、连翘酯苷A、木樨草苷、芦丁5种有效成分的含量的同时测定。

Abstract

Objective: To establish a HPLC-MS/MS method for simultaneously determining five components (chronogenic acid

phillyrin

forsythoside A

luteoloside

rutin) in Shuanghuanglian tablets. Method: Waters Acquity BEH C18column (2.1 mm×100 mm

1.7 μm) was adopted and eluted with the mobile phase of acetonitrile and 0.1% formic acid/water in a gradient mode at a flow rate of 0.4 mL·min-1. The analytes were detected by tandem mass spectrometry with the electrospray ionization (ESI) source in the multiple reaction monitoring (MRM) mode. Result: Ten compounds showed a good linear relationship (r>0.999);the precisions

repeatabilities and stabilities of the method were good

and the average recoveries were between 96% and 104.67% with the relative standard deviations of no more than 3%. Conclusion: The established method is highly repeatable

rapid and accurate and can be used for the determination of five effective components in Shuanghuanglian tablets.

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