Objective: To establish a HPLC method for the determination of quercetin and kaempferol in Dicranopiers dichotoma

and to determine the content of quercetin and kaempferol in D. dichotoma from different areas. Method: Different volume fractions of methanol and ethanol were chosen as the extraction solvents for quercetin and kaempferol. They were extracted by ultrasonic extraction and refluxing process and determined by HPLC. Diamonsil C18 column (4.6 mm×200 mm

5 μm) was adoped

with the mixture of methanol-0.1%H3PO4 water (54:46) as the mobile phase. The flow rate was 1.0 mL·min-1

and the column temperature at 30℃

the detection wavelength was set at 366 nm. Result: Refluxing process with 80% methanol was proved to be the optimal extraction method for quercetin and kaempferol

and the content of quercetin and kaempferol in D. dichotoma from Yingtan of Jiangxi Province was more than that from other areas. This method had a good separation performance for quercetin and kaempferol in D. dichotoma

and the linear range were 1.051-16.24

0.505-8.08 μg

respectively. Conclusion: This method is accurate

sensitive

stable and reliable

and can be used in the quality control of D. dichotoma. The contents of quercetin and kaempferol in D. dichotoma of different origins were significantly different.