HPLC同时测定灯盏细辛提取物中3种有效成分的含量

王舒

doi:10.13422/j.cnki.syfjx.2015190072

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HPLC同时测定灯盏细辛提取物中3种有效成分的含量

化学与分析 | 更新时间：2024-01-04

- HPLC同时测定灯盏细辛提取物中3种有效成分的含量
- HPLC Determination of Three Active Components in Extract of Erigerontis Herba
- 中国实验方剂学杂志 2015年21卷第19期页码：72-74
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.2015190072
  中图分类号： O657.72;R284.1
- 纸质出版日期：2015
- 稿件说明：
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王舒. HPLC同时测定灯盏细辛提取物中3种有效成分的含量[J]. 中国实验方剂学杂志, 2015,21(19):72-74.

WANG Shu. HPLC Determination of Three Active Components in Extract of Erigerontis Herba[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(19): 72-74.
王舒. HPLC同时测定灯盏细辛提取物中3种有效成分的含量[J]. 中国实验方剂学杂志, 2015,21(19):72-74. DOI： 10.13422/j.cnki.syfjx.2015190072.

WANG Shu. HPLC Determination of Three Active Components in Extract of Erigerontis Herba[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(19): 72-74. DOI： 10.13422/j.cnki.syfjx.2015190072.

摘要

目的:建立同时测定灯盏细辛提取物中野黄芩苷

5-二-O-咖啡酰奎宁酸和3

4-二-O-咖啡酰奎宁酸的含量测定方法。方法:采用高效液相色谱法

大连依利特C18色谱柱(4.6mm x 250 mm

5 μm)

流动相乙腈-0.1%磷酸(22:78)

检测波长330 nm

流速1 mL·min-1

柱温35℃。结果:野黄芩苷

5-二-O-咖啡酰奎宁酸和3

4-二-O-咖啡酰奎宁酸分别在0.032 7~1.308 0 μg(r=0.999 9)

0.048 2~1.928 0 μg(r=0.999 9)

0.058 3~2.332 0 μg(r=0.999 8)呈良好的线性关系

加样回收率分别为99.74%(RSD=1.9%)

100.23%(RSD=1.4%)

99.33%(RSD=1.3%)。结论:本法简便、快捷

结果准确、重复性好

可用于灯盏细辛提取物中3种有效成分的含量测定。

Abstract

Objective: To establish a method for determination of scutellarin

5-dicaffeoylquinic acid (3

5-DCQA)

4-dicaffeoylquinic acid (3

4-DCQA) in extract of Erigerontis Herba. Method: The HPLC method was carried out on Dalian Elite C18 column (4.6 mm×250 mm

5 μm) evaluated with acetonitrile-0.1% phosphoric acid (22:78) as mobile phase

the flow rate was 1 mL·min-1

the temperatue of column was at 35℃

the detection wavelength was at 330 nm for UV detection. Result: The calibration curves were linear in the range of 0.032 7 to 1.308 0 μg (r=0.999 9) for scutellarin

0.048 2 to 1.928 0 μg (r=0.999 9) for 3

5-DCQA

0.058 3 to 2.332 0 μg(r=0.999 8) for 3

4-DCQA

respectively. The average recoveries were 99.74% with RSD 1.9%; 100.23% with RSD 1.4% and 99.33% with RSD 1.3% respectively. Conclusion: The assay demonstrated that the method was simple

it had adequate accuracy and selectivity to quantify the three active components in extract of Erigerontis Herba.

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