Objective: To study excretion characteristics of senkyunolide I in urine and bile by intravenous injection and intragastric administration

respectively. Method: Rats were intravenous injected and intragastric administrated with senkyunolide I at a dose of 72 mg·kg-1

respectively.The urine and bile were collected at different time points.The concentrations of senkyunolide I in urine and bile were measured by HPLC-VWD

detection wavelength was set at 278 nm

mobile phase consisted of acetonitril-water (containing 0.1% formic acid) for gradient elution. Result: After intragastric administration and intravenous injection

cumulative excretion of senkyunolide I in 48 h were (179.8±33.68)

(264.8±87.28) μg in urine

cumulative excretion percentages were calculated to be (0.77±0.15)% and (1.35±0.26)%

respectively;cumulative excretion of senkyunolide I in 36 h were (359.4±75.66)

(426.3±140.90) μg in bile

cumulative excretion percentages were calculated to be (1.22±0.49)% and (1.72±0.59)%

respectively. Conclusion: Prototype is not main elimination form of senkyunolide I in rats' urine and bile

its speed of excretion is fast.