Objective: To explore the regulating effects of Clausenae Lansii Folium extracts on cytoskines level of type Th1/Th2 in inflammation reaction on asthmatic rats. Method: Forty-eight healthy male rats were randomly divided into six groups:normal control group

asthma model group

dexamethasone group(1.5 mg·kg-1·d-1)

Clausenae Lansii Folium extracts groups of low

medium and high doses (520

1 040

2 080 mg·kg-1·d-1). Except normal control group

all the other groups were sensitized with ovalbum by subcutaneous injection on the first and the seventh day. From the fifteenth day

rats in various administering groups received atomization treatment 0.5 hour after gavage 1 hour for 1 week. The normal control group and asthma model group were treated with normal saline instead. The levels of nitric oxide (NO)

leukotriene D4(LTD4)

interleukin (IL-4)

interferon-γ (IFN-γ) in serum were measured by ELISA

and NO in lung tissue was detected by nitrate reductase method. The expression of IL-4

IFN-γ mRNA in lung tissue was measured by RT-PCR. The pathological changes in lung tissue were observed. Result: Compared with the normal group

levels of NO

IL-4 and LTD4 in serum of rats in model group were significantly higher

and IFN-γ content was lower. NO content in lung tissues was also significantly higher

IL-4 mRNA expression was significantly higher

and IFN-γ mRNA was significantly lower (P<0.01). Compared with the model group

all of Clausenae Lansii Folium groups of low

medium and high dose could significantly reduce NO and IL-4 levels in serum

and increase IFN-γ level (P<0.05

P<0.01). Clausenae Lansii Folium groups of high

medium doses could reduce the level of NO in lung tissues of the asthma rats. Clausenae Lansii Folium high dose group could significantly reduce the level of LTD4 in serum of asthma rats (P<0.05

P<0.01). All of Clausenae Lansii Folium groups of low

medium and high dose could significantly reduce IL-4 mRNA expression and increase IFN-γ mRNA expression (P<0.05

P<0.01). Conclusion: The extracts of Clausenae Lansii Folium are effective in treatment of inflammation reaction in asthmatic rats by adjusting Th1/Th2 balance

and reducing the infiltration of inflammation cells.