Objective: To study the effect of drug-containing serum with modified Shengmai decoction on cell proliferation

cell cycle and migration of non small cell lung carcinoma(NSCLC)H460 cells

and investigate its molecular mechanisms. Method: Drug-containing serum with modified Shengmai decoction was prepared by administering the rabbits with 18 g·kg-1 crude drug ig

and blank control serum was prepared with the same volume of normal saline(NS) ig. Both serum was regarded as a concentration of 100%. H460 cells were cultured in vitro and cells in logarithmic growth phase were collected. 10% control serum group

2.5%

5%

10% drug-containing serum groups and 1 mg·L-1 DDP group were established. Cell density was adjusted to 3 000 cells/mL. After serum action for 24

48 hours

four methyl azo blue(MTT) method was used to measure the proliferation inhibition rate. Cell density was then adjusted to 5×105 cells/mL

and after serum action for 24 hours

flow cytometry was used to test cell cycle

scratch assay was taken to observe cell migration

and Western blot method was used to measure the expression changes of E-cadherin(E-cad) and Vimentin. Result: Compared with the blank control group

drug-containing serum with modified Shengmai decoction had higher cell proliferation inhibition rate (P<0.01)

higher cells percentage in S phase(P<0.01)

longer wound scratch distance

higher protein content of E-cad and lower Vimentin (P<0.01) after serum action for 24 hours. Conclusion: Modified Shengmai decoction has an inhibition effect on cell H460 mainly by inhibiting cells in phase S. Modified Shengmai decoction can inhibit the metastasis of H460

whose mechanism may be associated with intervening the epithelial-mesenchymal transformation.