京万红组方成分体外抗白色念珠菌抑菌效应探讨

张世川; 李亚楠; 魏雪仙; 国大亮; 刘岩; 赵宇; 刘玉璇

doi:10.13422/j.cnki.syfjx.2015190111

您当前的位置：

首页 >

文章列表页 >

京万红组方成分体外抗白色念珠菌抑菌效应探讨

药理 | 更新时间：2024-01-04

- 京万红组方成分体外抗白色念珠菌抑菌效应探讨
- Anti-bacterial Effects of Ingredients of Jingwanhong Prescription Against
- 中国实验方剂学杂志 2015年21卷第19期页码：111-115
- 作者机构：
- 作者简介：
- 基金信息：
  
  科技型中小企业发展专项(xqk2010-008)
- DOI：10.13422/j.cnki.syfjx.2015190111
  中图分类号： R285
- 纸质出版日期：2015
- 稿件说明：
移动端阅览
张世川, 李亚楠, 魏雪仙, 等. 京万红组方成分体外抗白色念珠菌抑菌效应探讨[J]. 中国实验方剂学杂志, 2015,21(19):111-115.

ZHANG Shi-chuan, LI Ya-nan, WEI Xue-xian, et al. Anti-bacterial Effects of Ingredients of Jingwanhong Prescription Against [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(19): 111-115.
张世川, 李亚楠, 魏雪仙, 等. 京万红组方成分体外抗白色念珠菌抑菌效应探讨[J]. 中国实验方剂学杂志, 2015,21(19):111-115. DOI： 10.13422/j.cnki.syfjx.2015190111.

ZHANG Shi-chuan, LI Ya-nan, WEI Xue-xian, et al. Anti-bacterial Effects of Ingredients of Jingwanhong Prescription Against [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(19): 111-115. DOI： 10.13422/j.cnki.syfjx.2015190111.

摘要

目的:研究京万红组方成分中盐酸小檗碱、黄芩苷及冰片的体外抗白色念珠菌作用。方法:以白色念珠菌为研究对象

K-B纸片扩散法分别测定50

100

150 g·L-1的盐酸小檗碱、黄芩苷、冰片及制霉菌素药液抑菌圈直径;采用试管双倍稀释法和棋盘法

测定盐酸小檗碱、黄芩苷、冰片、制霉菌素分别最低抑菌浓度(MIC)和最低杀菌浓度(MBC)

测定盐酸小檗碱、黄芩苷、冰片两两联用及3种组分联用的MIC

计算出联合抑菌分数(FIC)。结果:盐酸小檗碱、黄芩苷、冰片MIC分别为160

1 280

320 mg·L-1;MBC分别为640

20 480

640 mg·L-1。盐酸小檗碱与黄芩苷联用FIC指数0.5

为协同作用;黄芩苷与冰片联用FIC指数为0.625

为相加作用;盐酸小檗碱与冰片联用FIC指数为0.5

为协同作用。3种组分药物联合使用FIC指数为0.375

有一定的协同作用。结论:盐酸小檗碱、黄芩苷、冰片对白色念珠菌均有抑制作用

且3种组分药物联合使用具有一定的协同作用。

Abstract

Objective: To study the effect of anti-Candida albicans of Jingwanhong constituents of berberine hydrochloride

baicalin and borneol in vitro. Method: The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of berberine hydrochloride

baicalin

borneol (50

100

150 g·L-1) were determined by two-fold dilution method and checkboard method. The anti-bacterial effects of the combined three drugs were measured and the combined inhibitory fraction (FIC) was calculated. Result: The MIC of berberine hydrochloride

baicalin

borneol was 160

1 280

320 mg·L-1

and the MBC was 640

20 480

640 mg·L-1

respectively. Baicalin and berberine hydrochloride combined showed the synergistic effect with FIC index of 0.5.The FIC of baicalin and borneol combined was 0.625

which showed the additive effect.The berberine hydrochloride and borneol combined showed the synergistic effect with FIC index was 0.5.Three-drug combination had a certain synergy with the FIC index of 0.375. Conclusion: Three constituents of berberine hydrochloride

baicalin

borneol could inhibit C. albicans

and three drugs combined showed a certain synergy.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

三黄喷雾剂体外透皮吸收

HPLC测定炎可宁片中盐酸小檗碱、黄芩苷和汉黄芩素的含量

HPLC同时测定葛根芩连微丸中葛根素、甘草苷、黄芩苷、盐酸小檗碱的含量

黄芩苷-冰片联合应用对表皮葡萄球菌的抑菌作用