Objective: To observe the effect of Danggui Shuangshen Hamayou tablets (DSHT) in regulating the endocrine system of ovariectomized rats. Method: Totally 50 ovariectomized female rats were randomly divided into five groups:model group

Livial group and DSHT high

middle and low dose groups (0.6

0.3

0.15 g·kg-1)

with 10 in each group. Another 10 were included into the sham group. Each treatment group was fed with the corresponding liquid. Model group and sham group were orally given normal saline for 30 days. Animals were killed

rat brain

uterus and adrenal were collected to calculate the viscera index

and detect estradiol(E2)

luteinizing hormone (LH)

follicle-stimulating hormone(FSH) with content kit. Norepinephrine(NE)

dopamine (DA)

5-hydroxytryptamine(5-HT) and 5-hydroxyindole acetic acid(5-HIAA) content were tested by chemical fluorescence. Result: Compared with the sham group

the model group showed significantly lower weight

serum E2

uterus index

adrenaline index

NE

DA content (P<0.05

P<0.01) and higher LH

FSH

5-HT

5-HIAA content (P<0.05

P<0.01). DSHT could significantly increase the weight of rats in the model group

each DSHT group could significantly increased serum E2 levels in rats (P<0.05)

with no effect on LH

FSH. Each treatment group showed significantly increase in the lowered uterus index after castration (P<0.05)

and high dose of DSHT could increase the lowered adrenal index after castration (P<0.05). Each group showed significantly reduction in the increased 5-HT

5-HIAA content in ovariectomized rats (P<0.05). The high-dose and the middle-dose of DSHT could significantly elevated the reduced NE

DA levels in rats after castration (P<0.05). Conclusion: DSHT can regulate reproductive endocrine hormones and hypothalamic neurotransmitters.