Objective: To investigate the therapeutic effect of Huiru Yizeng I for treating hyperplasia of mammary glands

and screen out the best prescription by pharmacodynamic experiment. Method: Totally 120 rats were randomly divided into 15 groups (n=8). Except for the normal group

the other groups were intramuscularly injected with estradiol benzoate (0.5 mg·kg-1) for 25 days and then progesterone (5 mg·kg-1) for five more days to establish the mammary gland yperplasia model. The rats were treated with different drugs for four weeks. The serum estradiol (E2)

progesterone (P)

prolactin (PRL)

folliclestimulating hormone (FSH)

luteinizinghormone (LH) were determined by ELISA. The pathomorphological changes in mammary tissues of each group were observed under microscope. Result: Compared with the normal group

the model group showed significant increases in serum E2

P

PRL

FSH

LH content (P<0.05) and the numbers of lobules

alveoli and secretions. Compared with the model group

serum hormones of rats in each treatment values were lower (P<0.05). Compared with the other groups

group 8 showed significantly decreases in the serum levels of estradiol(P<0.05)

prolactin

follicle stimulating hormone

progesterone

luteinizing hormone (P<0.05) and reduction in the numbers of lobules

alveoli and secretions. Conclusion: Compound Huiru Yizeng I could significantly relieve the disorder of serum hormones

has significant therapeutic effect on hyperplasia of mammary glands

in which Hordei Fructus Germinatus

Prunellae Spica

Scrophulariae Radix

Fritillariae Thunbergii Bulbus

Laminariae Japonicae Thallus

Ostreae Concha

Cremastrae Pseudobulbus Pleiones Pseudobulbus are combined as the optimal prescription

with the most significant rapeutic effect on hyperplasia of mammary glands in rats.