Objective: To explore effects of different purification technologies on water extract of Glycyrrhizae Radix et Rhizoma. Method: Four kinds of technologies of ZTC 1+1 Ⅱ natural clarifying agents

chitosan and microfiltration combined with DA-201 macroporous resin respectively

in addition microfiltration-ultrafiltration were all used for purification of water extract of Glycyrrhizae Radix et Rhizoma.Retention rates of index components

physical and chemical parameters

and HPLC fingerprint were all applied to analyze applicability of different technologies. Result: Index components were reduced with combination technologies

which effectively removed macromolecules in water extract at the same time.Microfiltration technology in removing impurity was the best among the primary purifications.Similarity of purified liquid with microfiltration-ultrafiltration technology compared with original liquid was 0.886

which had minimal effect on liquid components among four purification technologies. Conclusion: Microfiltration-ultrafiltration combination technology might be a better method for separation and purification of water extract of Chinese medicines.This method avoids pollution of organic reagents on water extract of Chinese medicines

which is suitable for industrialized continuous production for easy operating and cost saving.