Objective: To develop a HPLC method for analyzing the content of doliroside B in total saponins from Dolichos appendiculatus roots

and to investigate intestinal absorption characteristics of doliroside B. Method: Ultimate AQ-C18 column (4.6 mm×250 mm

5 μm) was used with a mobile phase consisted of water-acteonitrile containing 0.1% acetic acid for gradient elution at a flow rate of 0.5 mL·min-1

detection wavelength was 215 nm and an injection volume was 20 μL. Result: Good linearity was obtained over the concentration range of 60-210 mg·L-1 with the correlation coefficient of 0.999 9.The mean recoveries were between 95% and 105%.The content of doliroside B in four batches of total saponins extract from D. appendiculatus roots were all above 80%.In addition

the absorption rate constant of doliroside B in small intestine was (0.018 3±0.001 1) h-1 and cumulative absorption percentage in 5 h was (9.49±0.26)%. Conclusion: This HPLC method is simple and accurate for quality control of doliroside B.Doliroside B is badly absorbed in small intestine.