何首乌中核苷类成分QTRAP UPLC-MS/MS分析

徐力; 罗益远; 刘娟秀; 刘训红; 房克慧; 兰才武; 侯娅; 马阳

doi:10.13422/j.cnki.syfjx.2015200055

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何首乌中核苷类成分QTRAP UPLC-MS/MS分析

化学与分析 | 更新时间：2024-01-04

- 何首乌中核苷类成分QTRAP UPLC-MS/MS分析
- Analysis of Nucleosides and Nucleobases in Polygoni Multiflori Radix by UPLC-MS/MS
- 中国实验方剂学杂志 2015年21卷第20期页码：55-58
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技支撑计划项目(2011BAI13B04);江苏高校优势学科建设工程资助项目(ysxk-2014)
- DOI：10.13422/j.cnki.syfjx.2015200055
  中图分类号： O657.63;R284.1
- 纸质出版日期：2015
- 稿件说明：
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徐力, 罗益远, 刘娟秀, 等. 何首乌中核苷类成分QTRAP UPLC-MS/MS分析[J]. 中国实验方剂学杂志, 2015,21(20):55-58.

XU Li, LUO Yi-yuan, LIU Juan-xiu, et al. Analysis of Nucleosides and Nucleobases in Polygoni Multiflori Radix by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 55-58.
徐力, 罗益远, 刘娟秀, 等. 何首乌中核苷类成分QTRAP UPLC-MS/MS分析[J]. 中国实验方剂学杂志, 2015,21(20):55-58. DOI： 10.13422/j.cnki.syfjx.2015200055.

XU Li, LUO Yi-yuan, LIU Juan-xiu, et al. Analysis of Nucleosides and Nucleobases in Polygoni Multiflori Radix by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 55-58. DOI： 10.13422/j.cnki.syfjx.2015200055.

摘要

目的:建立同时测定何首乌中10种核苷类成分的QTRAP UPLC-MS/MS分析方法

分析比较生何首乌和制何首乌中核苷类成分的差异。方法:采用QTRAP UPLC-MS/MS技术

Waters Atlantis T3色谱柱(2.1 mm×150 mm

3 μm)

流动相甲醇-5 mmol·L-1乙酸铵(含0.1%冰乙酸)

0.4 mL·min-1梯度洗脱

采用正离子多反应监测模式测定何首乌商品药材中10种核苷类成分的含量。结果:10种核苷类成分具有良好的线性关系

相关系数均> 0.99;何首乌商品药材中核苷类成分尿苷、腺嘌呤、鸟苷、胞苷含量均较高;生何首乌与制何首乌中核苷类成分含量差异明显。结论:该方法简便、灵敏、准确。该研究为何首乌药材内在质量的评价和控制提供可靠的检测方法。

Abstract

Objective: To develop a UPLC-QTRAP-MS/MS analysis method for the simultaneous determination of 10 nucleosides and nucleobases in Polygoni Multiflori Radix

and analyze the differences of nucleosides and nucleobases in raw and prepared Polygoni Multiflori Radix. Method: QTRAP UPLC-MS/MS technology was performed on a Waters Atlantis T3 column (2.1 mm×150 mm

3 μm)

eluted by a mobile phase of methanol-5 mmol·L-1ammonium acetate (0.1% acetic acid) at a flow rate of 0.4 mL·min-1. Ten kinds of nucleosides and nucleobases were analyzed in the positive ion multiple reaction monitoring (MRM) mode. Result: Ten nucleosides and nucleobases had good linearities

correlation coefficient > 0.99;the content of uridine

adenine

guanosine and cytidine was higher in Polygoni Multiflori Radix;there was significant difference in nucleoside compositions between raw and prepared Polygoni Multiflori Radix. Conclusion: The method is simple

sensitive

accurate

and can provide a reliable and effective technique for the quality control of Polygoni Multiflori Radix.

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